# Fish stress

## Problem description

Nine trucks with a couple hundred fish each. Each truck starts from the same location, but have a different destination across 3 years. For each truck, we have year, distance, and some water measurements. For a sample of fish, measures of stress were collected e.g. cortisol. Fish measurements were taken on different samples at times 0 (at hatchery), 2 (after arrival), 24, and 48 hrs.

### Question

How can we perform an exploratory analysis on these data?

## Consulting response

For an exploratory analysis, plot the fish measurements vs explanatory variables and times (or different plots for each time).

Calculate a mean and sd across the fish for each of the 36 combinations of truck and sample times. For an exploratory analysis, plot the mean (and sd) truck-time responses versus individual explanatory variables. Fit a regression model for the mean (and sd) truck-time responses versus individual explanatory variables.

Take a look at proportion of fish that died across the times as an additional measure of stress.

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